Metrohm 850 Professional IC Cation - Gradient - Manual (Page 79)

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5 Operation and maintenance
850 Professional IC Cation – HP-Gradient
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5.9 Sample degasser
5.9.1 Operation
If you are working with sample degassing, the longer "transfer time" (see
Ascertaining the transfer time, page 69) means that it is also necessary to
rinse for longer (with the subsequent sample). The rinsing time should be
at least 3 times the "transfer time" so as to minimize the carry-over
effects. The "transfer time" itself depends on the pump capacity, total
capillary volume and volume of gas removed (i.e. the amount of gas in the
sample).
Note
When using the sample degasser, the rinsing time extends by at least 2
minutes.
5.10 Injection valve
5.10.1 Protection
To prevent contamination of the injection valve, a 6.2821.120 inline filter
(see Chapter 3.11, page 37) should be mounted between the high pres-
sure pump and the pulsation damper.
5.11 Separation column
5.11.1 Separating efficiency
Which analysis quality can be attained, depends to a great extent on the
separating efficiency of the separation column used. The separating effi-
ciency of the selected separation column must be sufficient for the analy-
sis problems present. If difficulties occur, you should always first check the
quality of the separation column by recording a standard chromatogram.
You can find detailed information on the separation columns available
from Metrohm in the information sheet provided along with your separa-
tion column, in the Metrohm IC-Column Program (available via your
Metrohm agent) or in the Internet at
http://www.metrohm.com in the
product area Ion chromatography. You can request free information on
special IC applications in the corresponding "Application Bulletins" or
"Application Notes", which are available in the Internet at
http://
www.metrohm.com in the Applications area or via the Metrohm agent
responsible.