Bio-Rad Image Lab™ Software User Manual

Page 81

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Regression Methods

User Guide | 81

The following tables list the light type and filter to be used with various stains for

each application for the GS-900 densitometer.

Table 4. Imaging gels on the GS-900 calibrated densitometer

Stain

Light Type

Filter

Coomassie G-250 (colloidal)

Transmissive

Red

Coomassie G-250 (Bio-Safe™)

Transmissive

Red

Coomassie R-250

Transmissive

Red

Silver

Transmissive

Green

Copper

Transmissive

Red

Zinc

Transmissive

Green

Table 5. Imaging blots on the GS-900 calibrated densitometer

Detection Reagent

Light Type

Filter

Coomassie

Reflective

Red

Colloidal gold

Reflective

Green

Amido black

Reflective

Green

BCIP/NBT AP-substrate

Reflective

Green

4CN HRP-substrate

Reflective

Red

DAB HRP-substrate

Reflective

Green

Color blot

Reflective

Green

Table 6. Imaging X-ray film on the GS-900 calibrated densitometer

Film

Light Type

Filter

Blue film

Transmissive

Blue

Gray film

Transmissive

Red

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