Bio-Rad MicroRotofor™ Cell - Manual (Page 12)

6. Remove the focusing assembly from the running station. First, push
focusing assembly towards anode to dislodge the connector from the
cathode notch in the chassis. Then lift up gently on the cathode end and
remove the anode end (red).
7. With the row of sample loading ports facing up (sealing tape on loading
ports removed), position the focusing assembly in the harvesting station
(Figure 10). Two sides of the focusing chamber are flattened to correctly
orient the focusing assembly within the harvesting station and to help
align the harvesting needle array with the sealed harvesting ports of the
focusing chamber. DO NOT puncture the sealing tape covering the
harvesting ports.
Fig. 11. Placement of the focusing assembly into the harvesting station.
8. Taking care to not cover any of the loading ports with your fingers, and
using both hands, press down evenly and firmly on the electrode
assemblies so that all the needles penetrate the sealing tape and
harvesting ports simultaneously. At the same time and using the thumbs
of both hands, press the harvesting tray against the seal of the vacuum
assembly (Figure 12).
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