Bio-Rad MicroRotofor™ Cell - Manual (Page 3)

Section 1
Introduction
This kit was designed to familiarize you with the MicroRotofor™ Cell before
running your own sample. The setup and operation guides you through the
assembly and a complete fractionation and harvesting of a mixture of three
naturally colored proteins.
Note: For a detailed description of the MicroRotofor™ components, the
setup, and analysis of the results, please refer to the MicroRotofor™
instruction manual.
Section 2
Starter Kit Components
This kit contains:
• Bio-Lyte ® Ampholytes. 10 ml, pH range 3-10 – Catalog #163-1112
• Protein Sample, 1 ml – Catalog Number 170-2919
Phycocyanin, 2 mg: Blue protein, subunits pI range is 4.5–5.5
Hemoglobin, 2 mg: Red protein, subunits pI range is 6.0–7.5
Cytochrome c, 2 mg: Orange protein, subunits pI range is 8.0–9.0
• Focusing Chamber (1)
• Anode Membranes (Cation Exchange Membranes): 2 membranes
equilibrated in 15 ml electrolyte solution 0.1 M H
3
PO
4
• Cathode Membranes (Anion Exchange Membranes): 2 membranes
equilibrated in 15 ml electrolyte solution 0.1 M NaOH
• Harvesting Tray (1)
• Sample loading syringe, 3 ml (1)
• Electrolyte loading syringe, 10 ml (2)
Section 3
Required Equipment and Reagents
• MicroRotofor™ Cell with Instruction manual
• Power supply capable of 1 Watt constant power control or multistep
constant Voltage control, e.g. the PowerPac™ HV Power Supply
(Catalog N#164-5056)
1
10004399A.qxp 9/12/2005 2:08 PM Page 1