Bio-Rad MicroRotofor™ Cell - Manual (Page 14)

Section 5
Disassembly and Cleaning
5.1 Focusing Assembly
1. Using the assembly tool, loosen and remove the electrode assemblies
from the focusing chamber (Figure 14).
Fig. 14. Using the assembly tool to remove the anode assembly from the focusing
assembly.
2. Using the forceps, remove the ion exchange membranes from the
focusing chamber and immediately store them in deionized water or in
their respective electrolyte solution.
Note: The membranes cannot be allowed to dry out after they have been
equilibrated. The membranes may be stored in electrolyte or in distilled water
between runs. If they dry out, the membranes may crack and cause leakage
of electrolyte into the focusing chamber. Equilibrated membranes, if stored
properly, can be reused for 4–5 runs.
3. Rinse the electrode assemblies with deionized water.
4. Wash the focusing chamber. Place the focusing chamber in 2% SDS
solution overnight. Thoroughly rinse the focusing chamber with deionized
H
2
O to remove the SDS.
5.2 Harvesting Station
To maintain optimal harvesting performance, it is critical to wash the needle
array immediately after harvesting is complete. The entire harvesting station
and needle array may be detached from the chassis, washed with a mild
detergent, and rinsed with deionized water.
1. Disconnect the chassis from the vacuum source.
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