Protein expression profiles – Bio-Rad Rotofor® and Mini Rotofor Cells User Manual

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Protein Expression Profiles

Preparative liquid-phase isoelectric focusing with the Rotofor is an efficient

purification step for sample-sample comparisons of protein expression profiles.

Thoren K, Gustafsson E, Clevnert A, Larsson T, Bergstrom J, Nilsson CL (2002)
Proteomic study of non-typable Haemophilus influenzae.
J Chromatogr B Analyt Technol Biomed Life Sci. 782(1-2):219-26.

Kachman MT, Wang H, Schwartz DR, Cho KR, Lubman DM (2002)
A 2-D liquid separations/mass mapping method for interlysate comparison of ovarian cancers.
Anal Chem. 74(8): 1779-91.

Davidsson P, Paulson L, Hesse C, Blennow K, Nilsson CL (2001)
Proteome studies of human cerebrospinal fluid and brain tissue using a preparative
two-dimensional electrophoresis approach prior to mass spectrometry.
Proteomics. 1(3): 444-52.

Rosenkrands I, Weldingh K, Jacobsen S, Hansen CV, Florio W, Gianetri I, Andersen P
(2000)
Mapping and identification of Mycobacterium tuberculosis proteins by two-dimensional gel
electrophoresis, microsequencing and immunodetection.
Electrophoresis.21(5): 935-48

* Nilsson CL, Larsson T, Gustafsson E, Karlsson KA, Davidsson P (2000) Identification
of protein vaccine candidates from Helicobacter pylori using a preparative two-dimensional
electrophoretic procedure and mass spectrometry.
Anal Chem. 72(9): 2148-53.

* Wall DB, Kachman MT, Gong S, Hinderer R, Parus S, Misek DE, Hanash SM, Lubman
DM (2000)
Isoelectric focusing nonporous RP HPLC: a two-dimensional liquid-phase separation method
for mapping of cellular proteins with identification using MALDI-TOF mass spectrometry.
Anal Chem. 72(6): 1099-111.

Davidsson P, Nilsson CL (1999)
Peptide mapping of proteins in cerebrospinal fluid utilizing a rapid preparative
two-dimensional electrophoretic procedure and matrix-assisted laser desorption/ionization
mass spectrometry.
Biochim Biophys Acta. 1473(2-3): 391-9.

* A reprint of this article is available in the Rotofor technical folder.

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