Bio-Rad Rotofor® and Mini Rotofor Cells User Manual
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Bulletin #
Title
Detergents or Denarurants used
1518
Purification of Bacterial Eukaryotic Fusion Proteins Using the
Rotofor Cell
1517
Rotofor Fractionation of Intestinal Brush Border Membrane
Proteins
1514
Isolation of a Membrane Bound Immunoregulatory Molecule from
Metastatic Lymphoma Cells
1515
Preparation of Spinach Cold Acclimation Proteins for Gas Phase
Sequencing, Oligonucleotide Derivation and Monoclonal Antibody
Production
1475
Isolation of Monoclonal Antibodies to Phencyclidine from Ascite
Fluid
Section 13
Application for Preparative Two Dimensional
Electrophoresis System
Preparative electrofocusing in the Rotofor Cell is typically used for the initial
fractionation of proteins from crude mixtures. Following primary purification in the
Rotofor Cell, a final purification step may be needed to isolate a specific component.
Bio-Rad's Model 491 Prep Cell is a continuous elution polyacrylamide gel
electrophoresis device that is designed to be used as a secondary, and final
purification step following the Rotofor Cell. The following example illustrates the
usefulness of Bio-Rads unique "preparative two-dimensional electrophoresis system".
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