Bio-Rad Rotofor® and Mini Rotofor Cells User Manual

Page 36

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Isoform Resolution

Liquid-phase isoelectric focusing can be used as a method for effective resolution

of protein isoforms.

Stephenson K, Jensen CL, Jorgensen ST, Lakey JH, Harwood CR (2000)
The influence of secretory-protein charge on late stages of secretion from the Gram-positive
bacterium Bacillus subtilis.
Biochem J. 350 Pt 1: 31-9.

Kabir S (1995)
The isolation and characterisation of jacalin [Artocarpus heterophyllus (jackfruit) lectin]
based on its charge properties.
Int J Biochem Cell Biol. 27(2): 147-56.

Park YH, Lee SS (1994)
Identification and characterization of capsaicin-hydrolyzing enzymes purified from rat liver
microsomes.
Biochem Mol Biol Int. 34(2): 351-60.

Wang G, Bhattacharyya N, Wilkerson C, Ramsammy RA, Eatman E, Anderson WA
(1994)
Estrogen induced peroxidase secretion from the endometrial epithelium: a possible function
for the luminal enzyme.
J Submicrosc Cytol Pathol. 26(3): 405-14.

Chang YM, Lin S, Liao TH (1994)
Bovine pancreatic deoxyribonuclease F: isoelectric focusing, peptide mapping and primary
structure.
Biotechnol Appl Biochem. 19 ( Pt 1): 129-40.

Malle E, Hess H, Munscher G, Knipping G, Steinmetz A (1992)
Purification of serum amyloid A and its isoforms from human plasma by hydrophobic interac-
tion chromatography and preparative isoelectric focusing.
Electrophoresis. 13(7): 422-8.

Paliwal R, Costa G, Diwan JJ (1992)
Purification and patch clamp analysis of a 40-pS channel from rat liver mitochondria.
Biochemistry. 31(8): 2223-9.

Petrash JM, DeLucas LJ, Bowling E, Egen N (1991)
Resolving isoforms of aldose reductase by preparative isoelectric focusing in the Rotofor.
Electrophoresis. 12(1): 84-90.

Jimenez J, Dufresne M, Poirot S, Vaysse N, Fourmy D (1990)
Electric properties of photoaffinity-labelled pancreatic A-subtype cholecystokinin.
J Chromatogr. 511:333-9.

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