Bio-Rad Ligation and Transformation Module - Manual (Page 13)

Storage Instructions
The kit is shipped on blue ice. Open immediately upon arrival and store
reagent bags immediately at –20°C.
Safety Issues
Eating, drinking, smoking, and applying cosmetics are not permitted in the
work area. Wearing protective eyewear and gloves is strongly recommended.
Transformation reagent B contains dimethyl sulfoxide (DMSO, CAS #67-68-5),
an organic solvent. Handle with care and follow standard laboratory
practices, including wearing eye protection, gloves, and a laboratory coat
to avoid contact with eyes, skin, and clothing. If the solution comes into
contact with gloves, change the gloves. DMSO passes directly through
latex gloves, readily penetrates skin, and may result in the absorption of
toxic materials and allergens dissolved in the solvent. After handling, wash
hands and any areas that came into contact with the solution thoroughly.
Refer to MSDS for complete safety information.
Ampicillin may cause allergic reactions or irritation to the eyes, respiratory
system, and skin. In case of contact with eyes, rinse immediately with
plenty of water and seek medical advice. Wear suitable protective clothing.
Ampicillin is a member of the penicillin family of antibiotics. Those with
allergies to penicillin or any other member of the penicillin family of
antibiotics should avoid contact with ampicillin.
The E. coli HB101 K-12 strain is not pathogenic. However, handling of
E. coli HB101 K-12 requires the use of standard microbiological practices.
These practices include, but are not limited to, the following: (1) work surfaces
are decontaminated once a day after any spill of viable material; (2) all con-
taminated liquid or solid wastes are decontaminated before disposal; (3)
persons should wash their hands: (a) after they handle materials involving
organisms containing recombinant DNA molecules, and (b) before exiting
the laboratory; (4) all procedures should be performed carefully to minimize
the creation of aerosols and; (5) mechanical pipetting devices should be
used—mouth pipetting is prohibited.
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