Bio-Rad Ligation and Transformation Module User Manual
Page 8
When
Activity to Complete
Duration
At least 1 day prior to
Run a PCR reaction in thermal
3–4 h
starting the Ligation
cycler to amplify a
and Transformation
gene of interest
module
Electrophorese the PCR
1 h
products (optional)
Purify PCR products
0.5 h
At least 3 days prior to
Prepare LB and LB Amp
0.5 h
the transformation step
IPTG agar plates
At least 2 days prior to
Prepare LB broth
0.5 h
the transformation step
Streak
E. coli on a starter LB
5 min
agar plate
Grow
E. coli starter plate at 37°C
16+ h
As late as possible the
Inoculate starter culture
5 min
day before the
Incubate starter culture at
transformation step
37°C in a shaking
8+ h
water bath or incubator
Day of ligation step
Ligate PCR product
1 h
Note: Bolded steps use reagents from the Ligation and Transformation
module.
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