Bio-Rad Ligation and Transformation Module - Manual (Page 43)

11. Incubate tube at room temperature for 5–10 min.
12. Store the ligation reaction at –20°C. If you are proceeding directly to
transformation, pipet 5 µl of the ligation reaction into a microcentrifuge
tube labeled with your initials, the name of your gene, and
"transformation," and store it on ice until needed for the transformation.
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