Bio-Rad Ligation and Transformation Module User Manual
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electrophoresis. In addition, students must understand the principles of
PCR and be able to perform PCR reactions. Bio-Rad’s Biotechnology
Explorer program has a full range of kits to help teach basic skills in individual
laboratories.
What Is the Timeline for Completing the Ligation and
Transformation Protocol?
Before starting this activity, students must have already amplified a gene
of interest using PCR. In addition, the PCR product should be purified to
remove components of the amplification reaction that would otherwise
interfere with the ligation step.
The amount of time it takes to complete the ligation and transformation
protocols depends greatly on the level of your students and whether
additional/optional techniques and analyses are performed in addition to the
basic protocol. Steps using the Ligation and Transformation module are
highlighted in bold. Additionally, there are a few incubation steps that add
to the number of days it takes to complete the laboratory activity. A rough
guide is provided on pages 4 and 5.
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