Bio-Rad Biofuel Enzyme Kit - Manual (Page 10)

Quick Guide
Activity 6: Test the Ability of
Mushroom Extracts to Increase
the Reaction Rate
1. Write down the name of your mushroom
_______________________
2. Weigh out approximately 1 gram of
mushroom and put it into a mortar.
3. Add 2 ml of extraction buffer for every
gram of mushroom into the mortar. To
calculate the amount of extraction buffer
you need, multiply the weight (in grams)
of the mushroom by 2 and add that many
milliliters.
Weight of mushroom ____ g x 2 = _____ ml
4. Using a pestle, grind your mushroom to
produce a slurry.
5. Strain the solid particles out of your slurry
using a piece of filter paper or cheese
cloth into a 1.5 ml microcentrifuge tube.
Alternatively, if you have a centrifuge,
scoop the slurry into a 1.5 ml
microcentrifuge tube and then pellet the
solid particles by spinning at top speed
for 2 minutes. Note: You will need at
least 250 µl of extract to perform the
enzymatic reaction.
6. Label your cuvettes “1–6”. Only label on
the upper part of the cuvette face.
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Q
UICK GUIDE
Quick Guide
Extraction buffer
Label up here