Bio-Rad Biofuel Enzyme Kit - Manual (Page 9)

Please read and understand steps 7–9
fully before proceeding. These steps are
time sensitive!
7. Using a clean DPTP, pipet 750 µl of
enzyme into your 15 ml conical tube of
substrate labeled “High Concentration
Substrate”.
8. Using a clean DPTP, pipet 750 µl of
enzyme into your 15 ml conical tube of
substrate labeled “Low Concentration
Substrate”. START YOUR TIMER.
9. At the times indicated, use the correctly
labeled DPTP to remove 500 µl from the
15 ml centrifuge reaction tubes labeled
“High Concentration Substrate” and “Low
Concentration Substrate” and add it to
the appropriately labeled cuvette that
contains the stop solution.
10. Proceed with the analysis of your
samples. After you have finished your
analysis, rinse out the reaction tubes,
cuvettes, and DPTPs with copious water
and save them for later activities.
Note: Do not discard unused stock solutions.
They will be used for the next activity.
34
Q
UICK GUIDE
Quick Guide
Enzyme High concentration substrate
Enzyme Low concentration substrate
High
concentration
substrate reaction H1
1 min
H2
2 min
H3
8 min
Low
concentration
substrate reaction L1
1 min
L2
2 min
L3
8 min