Bio-Rad Biofuel Enzyme Kit - Manual (Page 6)

Quick Guide
Activity 4: Determine the Effect of
Enzyme Concentration on the
Reaction Rate
1. Label one 15 ml conical tube “Low
Concentration Enzyme”. Using a clean
DPTP, pipet 1 ml of buffer into the tube.
Wash out the DPTP with water. Pipet
1 ml of high concentration enzyme to
your tube labeled “Low Concentration
Enzyme” and mix. Wash out the DPTP
thoroughly with water.
2. Label three cuvettes “H1–H3” (for high
enzyme concentration time points) and
the remaining three cuvettes “L1–L3” (for
low enzyme concentration time points).
Only label on the upper part of the
cuvette face.
3. Using a clean DPTP, pipet 500 µl of stop
solution into each cuvette. Wash out the
DPTP thoroughly with water.
4. Label one clean DPTP with an “H” for
high enzyme concentration and a second
clean DPTP with an “L” for low enzyme
concentration.
31
Q
UICK GUIDE
Quick Guide
Label up here
Buffer Low
concentration
enzyme
High
concentration
enzyme
Stop Solution H1 H2 H3 L1 L2 L3