Iris Sample Processing StatSpin® CytoFuge 12 Versatile Cytocentrifuge System - Manual (Page 14)

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Cytofuge 12 operating parameters generally depend upon the size and specific gravity of the cells to
be concentrated onto the microscope slide. The operator should experiment with different settings to
achieve optimum performance for specific applications. The following are general guidelines:
Application Speed Range (RPM) Time Range
Cytology 400 to 650 2 to 4 minutes
Hematology 650 to 1500 4 to 8 minutes
Microbiology 1000 to 2000 4 to 10 minutes
For fragile cells, reduce speed.
For wet preparations, reduce time
For small particles (e.g. bacteria), increase speed and time
For specific application procedures, visit
www.statspin.com to access Application Notes for the
Cytofuge 12 cytocentrifuge.
Unloading
1. Upon completion of the cycle, the rotor will decelerate to a complete stop, a signal beep will
sound, and the cover latch will automatically release.
2. Lift the cover and carefully remove the rotor and transport to a biological hood.
3. Remove the rotor lid to access the sample concentrators
Programming
The Cytofuge ® 12 Cytocentrifuge can store up to 24 user-defined programs. Each program allows
independent settings for speed, time and acceleration. When a program number is selected, the display
will automatically update to show the speed, time, and acceleration associated with that program.
1. Press the Save button once. The displays will flash indicating that the instrument is in programming
mode.
2. Select the program number to be stored by depressing the up or down arrow keys to the right of the
Program display.
Note: Program 00 contains the default instrument settings and cannot be changed.
Programming can be cancelled at any time by pressing the Stop/Open button. The previous values for
that program number will be retained.
3. Adjust the speed and time values in a similar manner and select the desired acceleration rate.
4. Press the Save button a second time to store the values under the selected program number and exit
programming mode.