Boekel Scientific 136400-2 Shake 'N Bake - Manual

N2400040 REV 1 05/11
INSTRUCTIONS FOR INCUBATOR
-
SHAKER II
MODELS 136400, 136400S & 136400-2
(PLEASE READ THESE INSTRUCTIONS COMPLETELY BEFORE OPERATING)
PRODUCT INFORMATION
The Boekel Shake ‘N’ Bake (Incubator Shaker II) is de-
signed for scientists that prefer to use heat sealed bags
and Tupperware
® or Rubbermaid ® containers for hy-
bridizing and pre-hybridizing membranes in Southern
and Northern blotting techniques. The Boekel Shake ‘N’
Bake uses a microprocessor to control incubation tem-
perature. The unit’s shaking action can be adjusted from
approximately 8 to 25 strokes per minute or the unit can
be operated with no motion as a conventional incubator.
PRODUCT PERFORMANCE
The Boekel Incubator Shaker II has a chamber tem-
perature range of ambient plus 5°C to 70°C. The unit,
within the bounds of the shelves, has a chamber uni-
formity of ± 1.3° C at 65° and an “in the container” uni-
formity of ± 0.7°C at 65° (temperature uniformity of the
hybridization liquid in a heat sealed bag or Tupperware®
box). The Incubator Shaker II provides three shaking
shelves which can be loaded to a maximum combined
total weight of 1 Kg (2.2 lbs.) The shelves are spaced at
6.35 cm (2.5 inches) to allow the use of either heat
sealed bags or shallow Tupperware® containers.
Shelves may be removed to allow more space for larger
containers. Shelf shaking speed is controlled by an
on/off switch and a front panel mounted variable speed
control knob.
UNPACKING
1. Remove the Incubator Shaker II and tray set from
the carton.
2. Inspect for damage. Report all shipping damage
to the carrier immediately. Shipping damages are
covered by the carrier and repair and/or replace-
ment for shipping damages must be coordinated
through the carrier.
3. Retain all packaging material in the event that the
unit must be returned.
4. Completely fill out and return the self-addressed
Boekel Warranty Registration Card to Boekel
Scientific.
SET-UP
1. Place the unit in a clear, draft free environment.
Ensure that the surface on which the unit is
placed will withstand the radiated heat produced
by typical laboratory incubators. Ensure that the
side ventilation holes are not blocked.
2. Plug the power cord into a grounded receptacle
of appropriate voltage.
3. Place the heat sealed hybridization bag directly
on the shelf or on the supplied tray. Then place
the tray on the shelf. Note: Use of the trays may
diminish chamber uniformity due to temperature
stratification.
Tupperware
®
boxes up to 6.35 cm (2.5 inches) high may
be used on the lower two shelves. However, to prevent
shelf jamming, use a box no higher than 3.3 cm (1.3
inches) on the top shelf of Incubator Shaker II. Boxes
taller than 3.3 cm may be used on the top shelf only if
they can be placed to the right hand side of the shelf so
that they do not jam against the chamber ceiling on the
upward stroke of the shelf.
OPERATION
1. Turn the “Power” switch ON. Ambient chamber
temperature will be displayed on the digital read-
out.
2. Turn the “Shaker” switch on to start the shelf
rocking action. Increase the shaking speed by ro-
tating the “Shaker Speed” knob in the clockwise
direction.
Setting Temperature
The Temperature Controller has three buttons. When the
button on the left is depressed it will display “Target
Temperature”. When the left button is depressed simul-
taneously with the middle button, the “Target Tem-
perature” value is lowered. When the left button is de-
pressed simultaneously with the button on the right, the
“Target Temperature” is increased. When all buttons are
released, actual chamber temperature is displayed. In
the event of power loss, the Temperature Controller re-
tains last “Target Temperature” value. The flickering
green dot in the upper left corner of the Controller Dis-
play is the heat indicator light.