Olympus IX81-ZDC2 - Manual (Page 15)

12
IX81-ZDC2
TROUBLESHOOTING GUIDE
Under certain conditions, performance of the microscope may be adversely affected by factors other than defects. If problems
occur, please review the following list and take remedial action as needed.
If you cannot solve the problem after checking the entire list, please contact Olympus for assistance.
Problem Cause Remedy Page
a) Autofocusing is impossible. The AF port switching lever is not set to IN. Set it to IN. 8
Bubbles are present in the oil or water. Remove the bubbles. –
An objective other than specified is in
use. Use another objective or use manual
focusing.
4
The objective is mounted improperly. Screw in the objective firmly. –
The transmitted light bulb generates
infrared light. Close the shutter or turn off the light
bulb during AF.
5
A plastic bottom dish is in use. Use a glass bottom dish. 3
Electrical noise is interfering with op-
eration. Ground the IX2-UCB. Be also sure to
ground the ancillary equipment.
1
The glass bottom dish has a large
scratch. Use another glass bottom dish or use
manual focusing.
–
A phase contrast objective is in use. Use the specified objective. 4
Immersion oil (or water) of oil- or water-
Immersion objective is dried. Supply immersion oil (or water).
–
A water-immersion objective is used and
the interface between the water and
glass bottom dish is focused. Set the AF zone so that its center is
located on the interface between the
culture fluid and glass bottom dish and
the AF zone width is ±10 µm.
10
b) Autofocusing takes long time or
fails.
AF is set improperly. Set up AF properly.
(For the setting method, refer to the
instruction manual for the application
software.)
10
Electrical noise is interfering with op-
eration. Ground the IX2-UCB. Be also sure to
ground the ancillary equipment.
1
There is significant vibration in the in the
installed environment, or an anti-vibration
bench is not used. Be sure to use an anti-vibration
bench.
–
The specimen is vibrating. Lock the specimen firmly. Also confirm
that the stage center plate is free of
abnormality.
–
c) AF is applied to a position deviated
from the target position.
The offset value is input improperly. Enter the correct offset value. (For the
setting method, refer to the instruction
manual for the application software.)
10
Immersion oil (or water) of oil- or water-
Immersion objective is dried. Supply immersion oil (or water).
–
A water-immersion objective is used and
the interface between the water and
glass bottom dish is focused. Set the AF zone so that its center is
located on the interface between the
culture fluid and glass bottom dish and
the AF zone width is ±80 µm.
10
d) The specimen moves in the X-Y
directions during autofocusing.
The specimen is not locked. Lock the specimen by placing a
weight on the glass bottom dish, for
example. Also confirm that the stage
center plate is free of abnormality.
–
5