3B Scientific Monocular Microscope Model 400 with Vertical Viewing - Manual (Page 5)

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object slides and objective. Focus range: 15mm,
resolution of fine focussing adjustment: 0.002 mm
Tube: Head with double viewing capability, one
tube with 30° viewing angle, one with vertical view-
ing, head rotation 360°
Eyepiece: Pair of wide field eyepieces WF 10x 18
mm
Objectives: Revolver with 4 DIN achromatic objec-
tives 4x / 0.10, 10x / 0.25, 40x / 0.65, 100x / 1.25 oil
(with specimen protection)
Magnification: 40x, 100x, 400x, 1000x
Object stage: x-y cross table, 132 x 145 mm 2 , with
object guide and coaxial adjustment knobs per-
pendicular to the object stage, adjustment range
50 x 76 mm
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Illumination: Adjustable 6 V, 20 W halogen lamp
incorporated into the base, universal 85 to 265 V,
50/60 Hz power supply
Condenser: Abbe condenser N.A.1.25 NA 0.65 with
iris diaphragm , filter holder and blue filter, focus-
sed via rack and pinion drive
Dimensions: 291 x 214 x 415 mm³ approx.
Weight: 5,8 kg approx.
3. Unpacking and assembly
The microscope is packed in a molded styrofoam
container.
• Take the container out of the carton remove
the tape and carefully lift the top half off the
container. Be careful not to let the optical i-
tems (objectives and eyepieces) drop down.
• To avoid condensation on the optical compo-
nents, leave the microscope in the original pa-
cking to allow it to adjust to room tempera-
ture.
• Using both hands (one around the pillar and
one around the base), lift the microscope from
the container and put it on a stable desk.
• The objectives will be found within individual
protective vials. Install the objectives into the
microscope nosepiece from the lowest magni-
fication to the highest, in a clockwise direction
from the rear.
• Insert the eyepieces into the tubes.
4. Operation
4.1 General information
• Set the microscope on a level table.
• Place the object to be observed in the centre of
the specimen stage and clamp it to the object
guide.
• Connect the mains cable to the net and turn on
the switch to get the object illuminated.
• Make certain that the specimen is centered
over the opening in the stage.
• To obtain a high contrast, adjust the back-
ground illumination by means of the iris dia-
phragm and the variable illumination control.
• Rotate the nosepiece until the objective with
the lowest magnification is pointed at the
specimen. There is a definite “click” when each
objective is lined up properly.
NOTE: It is best to begin with the lowest power
objective. This is important to reveal general struc-
tural details with the largest field of view first.
Than you may increase the magnification as nee-
ded to reveal small details.
When 100x (oil) objec-
tive is chosen, objective oil must be dripped onto
the slide.
To determine the magnification at which you are
viewing a specimen, multiply the power of the
eyepiece by the power of the objective.
• Adjust the holding brake to give a suitable
degree of tightness in the focusing mechanism.
• Adjust the coarse-focusing-knob which moves
the stage up until the specimen is focused. Be
careful that the objective does not make con-
tact with the slide at any time. This may cause
damage to the objective and/or crack your
slide.
• Adjust the fine-focusing-knob to get the image
more sharp and more clear.
• Colour filters may be inserted into the filter
holder for definition of specimen parts. Swing
the filter holder out and insert colour filters.
• Use the knobs of the mechanical stage to move
the slide side-, back- and forwards. The vernier
provides accurate location of the specimen
area.
4.2 Changing the lamp and fuse
4.2.1 Changing the lamp
• Turn off the power switch, unplug the mains
plug and let the lamp cool down to avoid being
burnt.
• For safety reasons, remove the eyepiece.
• To change the lamp lay the microscope on its
back to reach the lid on the bottom side.
• Loosen screw C of the lamp socket and push it
outwards so that it is in the position shown in
Fig.1.
• Loosen screw A and open the cover.
• To remove the halogen lamp, use a cloth or
similar material. Do not touch the bulb with
the bare hand.