Luminex MAGPIX Hardware and Installation Manual - Manual (Page 92)

Possible Cause Corrective Action
The verification lot is expired. Use an unexpired bottle of verification
microspheres.
The verification microspheres have been diluted. Substitute undiluted verification microspheres.
The verification microspheres are photobleached. Use a different bottle of verification microspheres,
one which has been protected from light during
storage.
Possible Sample Probe Causes:
The sample probe height is incorrect. Perform an automatic sample probe height
adjustment.
The sample probe is clogged. Clogs on page 75.
The sample valve is faulty. Contact Technical Support.
Other Possible Causes:
There is carryover from the calibrators or the
previous assay. Carryover Problems on page 80.
There is air in the instrument. Verify the sample probe height. Use the software
to run a prime command three times, an alcohol
flush command twice, then a wash command
three times with distilled water.
Make sure the Drive Fluid coil is not pinched.
There is a problem internal to the instrument. Review the log of calibration reports. Check for
dramatic changes in temperature or voltage. If
any of these are present, contact Technical
Support.
Acquisition Failure
Acquisition failure can result from many of the same causes as calibration and verification
failure, in addition to sample and volume problems.
Possible Cause Corrective Action
Possible xMAP Microsphere Causes:
The xMAP microspheres are not fully suspended. Gently vortex the microtiter plate or resuspend
beads with a multichannel pipette to ensure that
the microspheres are present in the solution.
The wrong wells are selected for the xMAP
microspheres. Change the well setting in the software.
Troubleshooting Procedures
79