Luminex 100 User Manual Addendum Version 1.7 - Manual (Page 9)

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MAP Technology Luminex 100 User Manual Version 1.7 Addendum
PN 89-00002-00-143 Rev. B 3
Storing the Analyzer
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To prepare the system for storage:
1. Run a Sanitize command with 10% to 20% household bleach
solution.
2. Run a Sanitize command with distilled water.
3. Run four Wash commands with distilled water.
4. Remove the sample probe from the instrument, flush with
distilled water from the narrow end out through the larger end,
replace it in the sample arm and wrap the end with Parafilm.
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To prepare the system for first use after it has been in storage:
1. Turn on the Luminex 100 and XY Platform and watch for the
following indications that the instruments are responding
correctly:
• The light above the sample probe on the Luminex 100 and the
light next to the door of the XY platform are lit.
• The compressor starts in the Luminex 100. It is a low
rumbling sound.
• Place your hand behind the Luminex 100 to feel air coming
out of the rear fan. Place your hand on the left side of the XY
to feel air coming out of the XY.
• Observe movement of the syringe inside the front middle door
of the Luminex 100 shortly after the instrument is powered on.
2. Turn on the PC and start up the LDS software.
3. Complete a Warmup command, which will take 30 minutes.
4. Remove the Parafilm from the end of the sample probe.
5. After Warmup is complete, run three Back Flush commands,
three Drain commands, two Alcohol Flush commands, and three
Wash commands with distilled water. Make sure that the sheath
bottle or Luminex SD has a sufficient amount of sheath fluid and
that the waste container is empty. Verify that the pressure during
each of the maintenance commands reads between 6 and 9 psi in
the Device section on the right side of the LDS 1.7 software
window.
Sample Volume/
Sample Dilution
For optimal sampling efficiency and to reduce matrix effects,
concentrated biological fluids such as serum or plasma should be
diluted at least 1:5.