Multichannel Systems MEA2100-System Manual - Manual (Page 46)

MEA2100-System Manual
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Temperature Controller TC01 / 02
The MEA2100-System features a heating element with a Pt100 temperature sensor. If you connect
a temperature controller TCX to the heating element via D-Sub 9 connector, the heating element
guarantees constant temperature conditions for the biological sample, placed on the MEA.
Perfusion Cannula PH01
When using a perfusion system, the perfusion cannula with heating element and sensor PH01 can
be connected to a temperature controller TC02. This is very useful for general perfusion purposes,
whenever the fluid stream needs to be temperature controlled. Magnetic holders for the PH01
and a suction cannula and a steel plate fitting the headstage of the MEA2100 are available as
optional accessory.
For using pMEAs it is necessary to have a MEA2100-System equipped with a perfusion element.
The perfusion element is available for headstages with one 60 electrode MEA and headstages
with two 60 electrode MEAs. The perfusion element replaces the regular ground plate with
heating element. It is possible to change exchange the one element for the other, but the
exchange has to be done by MCS or a qualified distribution partner.
Setting up the Suction Port of the MEA2100-32-System
A suction port is integrated below the frame for the perforated MEA. Acute slices can be kept
in position and in good contact with the electrodes by applying suction through the perforated
MEA from below. It is a small sealed compartment that is connected to a tube. The red O-ring
for sealing this compartment is made of silicon. It has an inner diameter of 7 mm and a thickness
of 1 mm (Silicon 70, 7x1). By the tube, suction can be applied to the MEA from below either
manually via syringe, or via controlled vacuum pump CVP.