3 ph calibration – Thermo Fisher Scientific Alpha pH 550 User Manual

Page 21

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NOTES:

To exit calibration mode at any time during calibration, press

and

▼ keys simultaneously (escape). The pH Monitor returns

to the measurement mode and the old calibration values remain
active

• The calibration is always carried out in the units of

measurement (pH or mV), selected in setup mode

4.3 pH

Calibration

The pH Monitor is capable of calibration of up to 2 points using USA or NIST pH
buffer standards. All new calibration values will automatically override existing
calibration data. pH buffer values are referenced to 25

o

C. Make sure the

measurement mode is set to pH. (Refer Section 5.5 for switching measurement
modes). All pH buffer values have window of up to

± 1 pH tolerance during

calibration. Calibration error can be occurred if the measured pH value exceeds this
window. If you wish to abort the pH calibration, press

▲ and ▼ keys simultaneously

and the pH Monitor reverts to pH measurement mode.

4.3.1

For USA Standard Buffer

Make sure that the pH Monitor is set to accept USA standard buffer in the Setup
mode. (Refer Section 5.4 for Buffer Selection Settings) The factory default is USA
standard. It is recommended that you perform 2-point calibration at room
temperature (25

o

C), starting with the first buffer at pH 7.00 followed by any other

buffer value. (pH 4.01, 1.68, 10.01 or 12.45)

1-Point Calibration:

CAL

HOLD

ENT

CAL

pH

READY

HOLD

pH

CAL

CAL

mV

pH

HOLD

MEAS

PH

°C

ATC

READY

ENT

1

2

3

4

1

1

From pH measurement mode press CAL key to enter calibration mode as
described in section 4.2. The LCD shows ‘CAL PH’. Press ENT key to
begin calibration.

2

Place the electrode in pH 7.00 buffer. Immerse the temperature probe in
the buffer solution if ATC mode is enabled. Immerse the potential matching

pin in the buffer if symmetrical mode is enabled. The buffer annunciator

appears in LCD. Lower display shows pH 7.00 (USA standard buffer).

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