Agilent Technologies G1978B - Manual (Page 22)

22 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide
2 Verification
To verify sensitivity for 6120 Quad - G1978B Multimode, Positive SIM Modes
To verify sensitivity for 6120 Quad - G1978B Multimode, Positive
SIM Modes
Preparation
1 Transfer 1 mL of 5 ng/µL reserpine (Agilent G2423A) to a 50 mL volumetric
flask. Use a clean graduated pipette.
2 Dilute to the 50 mL mark with 75:25 methanol/water with 0.1% formic or
acetic acid.
3 Transfer 1 mL of the first dilution to a second 50 mL volumetric flask. Use a
clean graduated pipette.
4 Dilute to the 50 mL mark with 75:25 methanol/water with 0.1% formic or
acetic acid.
This provides the final 2 pg/µL reserpine concentration required for
performance verification.
5 Transfer approximately 1 mL of the second dilution to a vial for use in the
LC autosampler.
N O T E
The G6110A instrument does not support the multimode source without the Selection
valve. Use the option kit G2735H to enable the G6110A to work with the multimode source.
Table 1 6110 and 6120 Quad Performance Verification Summary Table
G1978B
ES Positive SIM Mode G1978B
APCI Positive SIM Mode
Sample Reserpine, 5 ng/µL Reserpine, 5 ng/µL
Concentration after dilution 2 pg/µL 2 pg/µL
Injection volume 5 µL 5 µL
Total sample amount injected 10 pg 10 pg
Sample order number G2423A G2423A
Solvent 75:25
methanol/water
with 0.1% formic or acetic acid
75:25
methanol/water
with 0.1% formic or acetic acid