Eppendorf Mastercycler pro - Manual (Page 27)

Operation
Mastercycler pro
English (EN)
27
5 Operation
5.1 Initial steps
Check the correct installation
Before the Mastercycler pro is started up for the first time, ensure the following:
• The device is correctly connected.
• The device is free of damage.
• Make sure that air can circulate freely around the ventilation slots on the rear of the device and around
the fan behind the feet.
5.1.1 Using the vapo.protect heated lid
Thevapo.protectheated lid features a heated flexible pressure unit. When the lid closes, the pressure unit
comes to rest lightly on the surface of the sample tubes. A liquid cushion in the pressure unit is then used to
create a pressure to ensure that the tubes are pressed uniformly into the thermoblock thereby protecting
the tube seals against evaporation. The heating process prevents condensation from forming in the upper
area of the tube when the reaction liquid is heated.
5.1.1.1 Closing the heated lid
Prerequisites
The thermoblock of the Mastercycler pro is loaded with sample tubes or a PCR plate (see Loading the
thermoblock on p. 50).
CAUTION! Damage to heated lid from sharp-edged sample tubes or objects.
 Use only tubes whose properties meet at least the requirements specified in these
operating instructions.
 Do not use any sharp-edged or sharp objects on the heated lid.
 Extra caution should be exercised when cleaning so as not to damage the heated lid.