Eppendorf Reference - Manual (Page 11)

10
The technical data is only valid when the quoted eppendorf pipette tips are used.
Tests carried out in accordance with ISO 8655 for piston-stroke pipettes with an
air cushion using a precision balance with evaporation trap approved by the
standardization authorities.
Number of determinations:
10; degassed, bidistilled water, 20 °C – 25 °C, constant to ± 0.5 °C; with pre-
wetted pipette tip; dispensing carried out on inner wall of vessel. If the place
where the pipette is used is at extremely high altitude, an adjustment must be
made in line with the ambient air pressure.
The pipettes in the Reference-Serie are piston-stroke pipettes that operate accor-
ding to the air-cushion principle.
The Reference-Serie consists of fixed-volume pipettes and pipettes with an
adjustable volume setting.
The control button is multi-functional. The function executed by the pipette
depends on how far its control button is pressed down.
Three steps are necessary to operate the pipette:
1. Measuring stroke
Press the control button down until the first stop. The desired volume of liquid
is dispensed or, when the button is released, aspirated.
2. Blow-out
Press the button down a little more until the next stop.
Any liquid remaining in the pipette tip is emptied.
3. Ejection
Press the button all the way down.
The pipette tip is ejected.
6 Function principle
Ex
20 °C
Eppendorf Reference® – Part A – Chapter 5 and 6