9 care / sterilization, 1 care, 2 sterilization – Eppendorf Reference User Manual
Page 18: Care / sterilization, Eppendorf reference® – part a – chapter 9
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9.1
Care
Depending on the frequency of use, all parts of the pipette should be cleaned from
time to time in a soap solution or should be carefully wiped clean using
isopropanol. They should then be rinsed in distilled water and dried.
The seals are maintenance-free and the pistons should be lubricated lightly (using
the grease for pipettes provided) when cleaned or replaced.
Severe contamination caused by the liquid entering the pipette can be removed
after the pipette has been disassembled (see Part B, Maintenance).
For information about replacing defective parts, please see Part B, Maintenance.
9.2
Sterilization
The Reference-Serie including the blank label provided (marked with a permanent
marker) is fully autoclavable at 121 °C for 20 minutes.
Before autoclaving, unscrew the pipette at the central junction by rotating about
one revolution. This enables steam to penetrate more easily into the pipette during
autoclaving.
After autoclaving, the pipette may have to be dried at room temperature. Retighten
the central junction only after the pipette has completely cooled.
The tip cone may have to be tightened again with the wrench (see part B, Mainte-
nance).
The Reference pipettes may be temporarily subjected to the UV light of a sterile
bench (≥ 254 nm).
9
Care / Sterilization
Eppendorf Reference® – Part A – Chapter 9