21 connecting the separation column – Metrohm 940 Professional IC Vario ONE/ChS/HPG User Manual
Page 56
3.21 Connecting the separation column
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940 Professional IC Vario ONE/ChS/HPG (2.940.1240)
￭
Start manual control in MagIC Net and select the high-pressure
pump: Manual
▶ Manual control ▶ Pump
– Flow: in accordance with column leaflet
– On
￭
Rinse the guard column with eluent for approx. 5 minutes.
￭
Use manual control in MagIC Net to stop the high-pressure pump
again: Off.
3.21
Connecting the separation column
The intelligent separation column (iColumn) is the heart of the ion chro-
matographic analysis. It separates different components according to their
interactions with the column. Metrohm separation columns are equipped
with a chip where their technical specifications and history (start-up, oper-
ating hours, etc) are stored.
NOTE
Information regarding which separation column is suitable for your
application can be found in the Metrohm IC Column Program, the
(Ion Chromatography product area) or obtained
directly from your representative.
You can find the separation columns and guard columns currently avail-
able from Metrohm in the Metrohm IC Column Program or on the Inter-
area. A test chromatogram and a leaflet are provided along with each col-
umn. You can find detailed information on special IC applications in the
corresponding "Application Bulletins" or "Application Notes", which
are available on the Internet at
in the Applica-
tions area or free of charge upon request from your responsible Metrohm
representative.
CAUTION
New separation columns are filled with solution and sealed with stop-
pers on both sides. Before inserting the column, ensure that this solu-
tion can be mixed with the eluent being used (follow information from
the manufacturer).