Bio-Rad Gene Pulser MXcell™ Electroporation System - Manual (Page 11)

11
Electroporation Conditions Are Known
Cell number >5 x 10 6
Gene Pulser MXcell Electroporation Guide
Check all well
sets after each
electroporation
for transfection
efficiency and
cell viability.
For an overview of
common analytical
methods, go to
pages 15–17.
Visit us on the Web at discover.bio-rad.com | Known Conditions: Exponential Setup — Whole Plate
Choose whole plate
preset protocols that
best match your
known conditions
and edit parameters
as needed, following
the instructions.
Use the top half of the electroporation plate to identify optimal voltage.
n
Enter the known capacitance value in well sets ABCD 1–12, or keep preset values
if unknown
n
Enter the known voltage (median voltage) value in well sets ABCD 4–6
n
Vary the voltage by 100 V increments around the median value in other well sets
n
Keep the preset values for all other parameters
Use the bottom half of the electroporation plate to identify optimal capacitance.
n
Enter the known voltage value in well sets EFGH 1–12
n
Enter the known capacitance (median capacitance) value in well sets EFGH 4–6
n
Vary capacitance by –10% around the median value in other well sets or use
preset values
n
Keep the preset values for all other parameters
Save the protocols under a new name.
1 2 3 4 5 6 7 8 9 10 11 12
A
DV
(V)
100 100 100 200 200 200 00 00 00 400 400 400
B
C
D
E
DC
(µF)
200 200 200 0 0 0 00 00 00 1,000 1,000 1,000
F
G
H
Exponential Waveform Protocols
Use whole plate preset protocols 96-well/Exp, Vgrad, Cgrad to identify
optimal voltage and capacitance.
Good results? Congratulations! Be rewarded for your work and share your protocol
with scientists worldwide. Visit www.bio-rad.com/genetransferprotocols/.
Would you like to further optimize? For manual programming, go to page 14.
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