Bio-Rad Gene Pulser MXcell™ Electroporation System - Manual (Page 18)

96-well/Exp 96 96 Use for initial optimal protocol Applies the same waveform
identification for many cell types across the whole plate
96-well/Sqr 96 96 Use for initial optimal protocol Applies the same waveform
identification for many cell types across the whole plate
Mixed 96-well/Exp, Sqr 96 96 Use for mixing different waveforms Exp: 20 V, 0 μF
by alternating rows of exponential Sqr: 20 V, 20 ms
(20 V/0 μF) and square waveforms
(20 V/20 ms)
Mixed 24-well/Exp, Sqr 24 24 Use for mixing different waveforms by Exp: 20 V, 0 μF, 1,000 Ω
alternating rows of exponential Sqr: 20 V, 20 ms, 1,000 μF, 1,000 Ω
(20 V/0 μF) and square waveforms
(20 V/20 ms)
24-well/Exp 24 24 Use for initial protocol setup for Same conditions for the
many cell types whole plate
24-well/Sqr 24 24 Use for initial protocol setup for Same conditions for the
many cell types whole plate
Opt 24-well/Exp, Sqr 24 24 Use with cell line with no protocol Exp: 10–40 V, 200–1,000 μF
reference; this protocol includes a Sqr: 10–40 V, –0 ms
range of common starting conditions
Uniform 24-well/Exp, Sqr 24 24 Use with a set of defined conditions Exp: 20 V, 0 μF, 1,000 Ω
to compare different cell lines and Sqr: 20 V, 20 ms, 1,000 μF, 1,000 Ω
electroporation of different molecules
within the same or different cell lines
12-well/Exp 12 12 Use for initial protocol setup for many Same conditions for the
cell types whole plate
12- well/Sqr 12 12 Use for initial protocol setup for many Same conditions for the
cell types whole plate
Opt 12-well/Exp, Sqr 12 12 Use with cell line with no protocol reference; Exp: 10–400 V, 200–00 μF
this protocol includes a range of common Sqr: 10–00 V, 1–2 ms
starting conditions
Uniform 12-well/Exp, Sqr 12 12 Use with a set of defined conditions to Exp: 20 V, 0 μF, 1,000 Ω
compare different cell lines and Sqr: 20 V, 20 ms, 1,000 μF, 1,000 Ω
electroporation of different molecules
within the same or different cell lines
Mixed 12-well/Exp, Sqr 12 12 Use for mixing different waveforms by Exp: 20 V, 0 μF, 1,000 Ω
alternating rows of exponential Sqr: 20 V, 20 ms, 1,000 μF, 1,000 Ω
(20 V/0 μF) and square waveforms
(20 V/20 ms)
Additional Preset Protocols Available
Gene Pulser MXcell Electroporation Guide
Visit us on the Web at discover.bio-rad.com | Additional Preset Protocols
Preset Protocols Application Parameters
See the Gene Pulser MXcell
Electroporation System
manual for more information.
Plate
Type
# of
Wells
Used
1