Bio-Rad Gene Pulser MXcell™ Electroporation System - Manual (Page 8)

Cell number >5 x 10 6
Use preset protocols Opt 96-well/Exp, Sqr to identify waveforms and
other initial conditions.
Gene Pulser MXcell Electroporation Guide
Unknown Electroporation Conditions
Check all well
sets after each
electroporation for
transfection efficiency
and cell viability.
For an overview of
common analytical
methods, go to
pages 15–17.
Good results? Congratulations! Be rewarded for your work and share your protocol
with scientists worldwide. Visit www.bio-rad.com/genetransferprotocols/.
Would you like to further optimize? Use the preset protocols on pages 9–10 ( if < x 10 6 cells)
or pages 11–12 (if > x 10 6 cells) ; for manual programming, go to page 14.
Square waveform Exponential waveform
1 2 3 4 5 6
A
200 V
2,000 µF
20 ms 20 V
2,000 µF
20 ms 00 V
2,000 µF
20 ms 20 V
0 µF
1,000
W
20 V
00 µF
1,000
W
20 V
70 µF
1,000
W
B
C
D
1 2 3 4 5 6 7 8 9 10 11 12
A
10 V
0 µF 200 V
0 µF 20 V
0 µF 00 V
0 µF 0 V
0 µF 400 V
0 µF 20 V
200 µF 20 V
20 µF 20 V
0 µF 20 V
00 µF 20 V
70 µF 20 V
1,000 µF
B
C
D
E
10 V
20 ms 200 V
20 ms 20 V
20 ms 00 V
20 ms 0 V
20 ms 40 V
20 ms 20 V
 ms 20 V
10 ms 20 V
1 ms 20 V
20 ms 20 V
2 ms 20 V
0 ms
F
G
H
S
q
ua
re
Exponential
Good for
first time
users!
Cell number <5 x 10 6
Use preset protocols Opt mini 96-well/Sqr, Exp to identify waveforms and
other initial conditions.
Visit us on the Web at discover.bio-rad.com | Unknown Conditions: Setup