Bio-Rad Gene Pulser MXcell™ Electroporation System - Manual (Page 14)

Once the best waveform is identified, use this tree to program the MXcell system to
improve other parameters.
Electroporation Protocols Decision Tree
Gene Pulser MXcell Electroporation Guide
Exponential Waveform Square Waveform
Optimize Capacitance
Manually program a capacitance
gradient (–10% increments)
around the value that yielded the
best results, keeping all other
parameters constant.
Optimize Voltage
Program a voltage gradient around
the value that yielded the best
results, keeping all other
parameters constant.
Optimize Voltage
Using the optimized capacitance
value, program a voltage gradient
(–10% increments) around the value
that yielded the best results,
keeping all other parameters constant.
Optimize Pulse Duration
Use the optimized voltage and keep all
other parameters constant. Vary pulse
values at  ms intervals around the
pulse that yielded the best
results (see protocols on page 10).
Optimize Resistance
Using the optimized capacitance and
voltage values, manually program a
resistance gradient (–10% increments)
around the values that yielded the
best results, keeping all other
parameters constant.
Optimize Number of Pulses
Use 2– shorter pulses that add
up to the pulse duration that yielded
the best results (see protocols
on page 10).
Initial Experimental Results
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