Bio-Rad Silver Stains - Manual (Page 10)

3) Individual gels. Prepare working solutions according to Table 4. To stain multiple gels, multiply the
solution volumes by the appropriate number of gels. The working solution volumes listed below are
recommended volumes — the actual required volume may vary depending on the dimensions of the
staining container used. For all cases, sufficient volumes of working solution should be prepared to
completely submerge the gel to be stained.
Table 4. Suggested working solution volumes for individual gels.
Working Solutions Reagents Individual Mini or Individual Large Gels
Criterion ™ Gels 16 x 16 cm or larger
(250 ml) (1 L)
Fixing Ethanol 100 ml 400 ml
Glacial acetic acid 25 ml 100 ml
Water To 250 ml To 1 L
Sensitizing SC (10×) 25 ml 100 ml
BRC (100×) 2.5 ml 10.0 ml
Ethanol 75 ml 300 ml
Water To 250 ml To 1 L
Staining SRC (50×) 5 ml 20 ml
Water To 250 ml To 1 L
Developing DBC (10×) 25 ml 100 ml
IDC (5,000×) 50.0 µl 200.0 µl
BRC (20,000×) 12.5 µl 50.0 µl
Water To 250 ml To 1 L
Stopping Glacial acetic acid 12.5 ml 50.0 ml
Water To 250 ml To 1 L
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