Bio-Rad Silver Stains - Manual (Page 9)

Table 3. Dodeca stainer volumes for less than 12 trays.*
Working Solutions Reagents Small Dodeca Stainer Large Dodeca Stainer
4–11 Trays 4–11 Trays
Per Tray Plus Per Tray Plus
Fixing Ethanol 200 ml 400 ml 300 ml 600 ml
Glacial acetic acid 50 ml 100 ml 75 ml 150 ml
Water To 500 ml To 1 L To 750 ml To 1.5 L
Sensitizing SC (10×) 50 ml 100 ml 75 ml 150 ml
BRC (100×) 5 ml 10 ml 7.5 ml 150 ml
Ethanol 150 ml 300 ml 225 ml 450 ml
Water To 500 ml To 1 L To 750 ml To 1.5 L
Staining SRC (50×) 10 ml 20 ml 15 ml 30 ml
Water To 500 ml To 1 L To 750 ml To 1.5 L
Developing DBC (10×) 50 ml 100 ml 75 ml 150 ml
IDC (5,000×) 100 µl 200 µl 150 µl 300 µl
BRC (20,000×) 25 µl 50 µl 37.5 µl 75 µl
Water To 500 ml To 1 L To 750 ml To 1.5 L
Stopping Glacial acetic acid 25 ml 50 ml 37.5 ml 75 ml
Water To 500 ml To 1 L To 750 ml To 1.5 L
*Please refer to page 6 for further details
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