Bio-Rad Silver Stains - Manual (Page 12)

10
Section 5
Troubleshooting Guide
Problem Cause Solution
Background dark or uneven Agitation too gentle. Increase agitation speed during
gel incubation and ensure gel is
completely submerged.
Dodeca stainer or staining Thoroughly clean Dodeca stainer
containers contain residue from or containers with soap and
prior cleaning or staining runs. water. Rinse only glass
containers with 50% nitric
acid, followed by thorough
water rinse to remove the acid.
Fingerprints or powder from Use powder-free laboratory
gloves cause staining artifacts. gloves.
Interfering components (glycine, Increase fixing time or use
SDS, etc.) not completely multiple fixing cycles to
removed from gel. completely wash interfering
compounds from gel.
Sensitizing working solution Prepare new sensitizing
improperly prepared. solution with BRC.
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