Bio-Rad Silver Stains - Manual (Page 6)

Section 3
Reagent Preparation
Additional Materials Needed
In addition to the components in the Dodeca silver stain kit, the following materials are required:
• Reagent-grade ethanol and glacial acetic acid
• Deionized, distilled, or high purity water. Water of ≥18 MΩ-cm resistivity is recommended
• Dodeca stainer (catalog #165-3400 or 165-3401), or glass or plastic staining trays
• When not using a Dodeca stainer, all steps in the procedure require agitation of the gel in solution — for
best results, a shaker table is recommended
Warnings and Precautions
Read each label in the kit before beginning the procedure.
Always wear powder-free laboratory gloves when handling gels to prevent protein contamination.
The image developer concentrate contains formaldehyde and should be used in properly ventilated areas.
Avoid contact with skin. In case of contact with eyes, flush with copious amounts of water and contact a
physician.
See Material Safety Data Sheets for additional information about each solution.
Dispose of waste material according to local regulations.
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