Bio-Rad Criterion™ Blotter - Manual (Page 12)

CAPS Buffer with 20 % Methanol
10 mM CAPS (3-(cyclohexylamino)-1-propane sulfonic acid), 20% v/v methanol, pH 11
From dry reagents:
Mix 2.21g CAPS in 600 ml of dd H
2
O, ADJUST the pH to 11.0 with NaOH, add
distilled deionized water (dd H
2
O) to 800 ml, add 200 ml Methanol.
CAPS Buffer with 10 % Methanol
10 mM CAPS (3-(cyclohexylamino)-1-propane sulfonic acid), 10% v/v methanol, pH 11
From dry reagents:
Mix 2.21g CAPS in 600 ml of dd H
2
O, ADJUST the pH to 11.0 with NaOH, add
distilled deionized water (dd H
2
O) to 900 ml, add 100 ml Methanol.
CAPS Buffer with 15% Ethanol
10 mM CAPS (3-(cyclohexylamino)-1-propane sulfonic acid), 15% v/v ethanol, pH 11
From dry reagents:
Mix 2.21g CAPS in 600 ml of dd H
2
O, ADJUST the pH to 11.0 with NaOH, add
distilled deionized water (dd H
2
O) to 850 ml, add 150 ml Ethanol.
2. Native gels
25 mM Tris, 192 mM glycine, pH 8.3
a) Use Premixed buffer concentrate solution:
100 ml of 10X Tris /Glycine buffer (catalog number 161-0734 1L bottles or catalog
number 161-0757 5L cube)
900 ml of dd H
2
O
b) From dry reagents:
Add 3.03 g Tris, 14.4 g glycine to distilled deionized water (dd H
2
O) then mix and
add dd H
2
O to a final volume of 1 liter.
3. Nucleic Acid gels
a) TBE (Tris-Borate EDTA)
89 mM Tris borate, 2 mM EDTA pH 8.3
100 ml of 10X TBE buffer (catalog number 161-0733 1L bottles or catalog number
161-0770 5L cube)
900 ml of dd H
2
O
b) TAE (Tris-Acetate EDTA)
40 mM Tris-Acetate 1 mM EDTA
20 ml of 50X TAE buffer (catalog number 161-0743 1L bottles or catalog number
161-0773 5L cube)
980 ml of dd H
2
O
10