Bio-Rad Criterion™ Blotter - Manual (Page 3)

Section 1
General information
1.1 Introduction
The Criterion Blotter is an electrophoretic transfer cell designed for use with Criterion
precast gels as well as hand cast gels. The Criterion Blotter cell is available with standard
wire platinum electrodes or with plate electrodes, which consist of a platinum-coated titani-
um anode and a stainless steel cathose. Transfers are performed with either set of electrodes
positioned 4.3 cm apart, using one or two gel holder cassettes positioned between the elec-
trodes. This allows generation of ahigh intensity electrical field for and efficient transfer when
used in combination with the PowerPac 200 Power Supply. Cooling is required for temper-
ature control and can be achieved with the sealed ice block included with the cell, or with the
optional cooling coil. The Criterion Gel Blot Assembly Tray provides for lab tidy assembly
of gel blot sandwiches and gel soaking, while minimizing the possibility of incorrect sandwich
assembly. The foller is useful to ensure proper contact and removal of trapped bubbles dur-
ing sandwich assembly.
1