Bio-Rad Criterion™ Blotter - Manual (Page 7)

h. Place a piece of filter paper on top of the membrane. Run the roller gently over the
top of the filter paper to remove any air bubbles trapped in the sandwich.
i. Wet a second fiber pad in the front compartment of the tray (where the membrane was
soaking) again using finger tips to completely saturate the pad with transfer buffer.
Then place the wet fiber pad on top of the second filter paper.
j. Lower the clamp-side of the cassette, and lock in the closed position (see figure).
Note: New fiber pads will create a tight fit when the cassette is closed. Subsequent use will
make the pads more compressible.
2.2 Begin Transfer
a. Move the locked cassette into the groove in the blotter tank, aligning the red side of
the card with the red electrode. Make sure the magnetic stirrer is free to move (see
figure).
b. After both cassettes are in place, add the remaining transfer buffer to the fill level
marked on the tank.
c. Put on the lid, plug the cables into the power supply, and run the blot. Refer to Section
3 for run times with various buffers.
d. Transfer at a constant voltage using the Bio-Rad PowerPac 200 which has current
capacity of 2A.
Upon completion of the run, disassemble the blotting sandwich and remove the
membrane for development. Clean the cell, fiber pads, and cassettes with multiple
rinses of deionized water.
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