Bio-Rad Criterion™ Blotter - Manual (Page 5)

Bio-Rad is not responsible for any injury or damage caused by the use of this instrument
for purposes other than for which it is intended or by modifications of the instrument not
performed by Bio-Rad or an authorized agent.
Section 2
Set Up and Basic Operation
Criterion Blotter Cell Assembly
2.1 Preparation for Blotting
1. Freeze ice block prior to preparation of blot assembly.
2. Prepare the transfer buffer. (See Section 3.3 for buffer formulation. Using buffer
pre-chilled to 4°C will improve heat dissipation.)
3. Cut membrane and the filter paper to the dimensions of the gel if necessary.
4. Equilibrate gel in transfer buffer for 15 minutes.
Note: Always wear clean gloves when handling the transfer cell, membranes, filter paper, or
gels to prevent contamination. (See Sectioin 3.2 for details.)
5. Set up transfer apparatus.
• Fill the Criterion Blotter tank with transfer buffer to about 50% of the fill volume.
• Place a magnetic stir bar inside the tank. (0.8–10 mm)
• Place the ice block in the ice block pocket in the back of the cell. Flip down the lever
to hold the ice block down.
• Alternatively, the optional cooling coil can be used. Connect it to an appropriate
recirculating water chiller and place it in the grooves in the back of the tank.
New Figure.
3