Bio-Rad Trans-Blot® Cell - Manual (Page 3)

Table of Contents
Section 1 Introduction ..................................................................................................1
1.1 Specifications .............................................................................................................1
1.2 Safety Instructions......................................................................................................2
Section 2 Trans-Blot Cell Assembly............................................................................4
2.1 Assembly of the Unit for Standard Transfers ............................................................4
2.2 High Intensity Field Option .......................................................................................7
2.3 Acidic Transfers .........................................................................................................8
Section 3 Transfer Conditions .....................................................................................8
3.1 General Guide for Transfer Buffers and Running Conditions ..................................8
3.2 Notes on Electrophoretic Transfer Conditions ..........................................................9
3.3 Buffer Formulation...................................................................................................11
Section 4 Strategies for Optimizing Electro-Elution ..............................................12
4.1 Optimizing Protein Transfer ....................................................................................12
4.2 Optimizing DNA and RNA Transfer.......................................................................13
Section 5 Choice of Blotting Membranes.................................................................14
5.1 Protein Blotting Membranes ....................................................................................14
5.2 DNA and RNA Blotting Membrane........................................................................14
Section 6 Troubleshooting Guide ..............................................................................15
Section 7 Product Information ..................................................................................22
Section 8 References ...................................................................................................22