Bio-Rad Trans-Blot® Cell - Manual (Page 9)

7. Addition of a standard stir bar will help to maintain even buffer temperature and ion dis-
tribution in the tank. Set the speed as fast as possible to keep ion distribution even.
8. Put on the lid, plug the cables into the power supply, and run the blot. Refer to Section 3
for run times and voltage setting with various buffers.
9. Upon completion of the run, disassemble the blotting sandwich and remove the mem-
brane for development. Clean the cell, fiber pads, and cassettes with laboratory detergent
and rinse well with deionized water.
6