Bio-Rad Immun-Blot® AP Colorimetric Kits - Manual (Page 2)

Table of Contents
Section 1 Preparation .................................................................... 1
1.1 Introduction ................................................................................ 1
1.2 Product Information.................................................................... 2
1.3 Materials Provided with the Immun-Blot Kit; Storage
and Stability of Kit Components ................................................
4
1.4 Safety Instructions ...................................................................... 5
1.5 Solutions ..................................................................................... 6
Section 2 Immun-Blot Assay ......................................................... 9
2.1 Experimental Strategy and General Recommendations ............. 9
2.2 Detailed Assay Procedure........................................................... 11
2.3 Detailed Color Development Procedure..................................... 13
Section 3 Troubleshooting............................................................. 16
3.1 Tests for Monitoring Reagent Activity....................................... 16
3.2 Troubleshooting Guide ............................................................... 17
Section 4 References ...................................................................... 21
LIT171D 1/8/99 1:39 PM Page B