Bio-Rad Immun-Blot® AP Colorimetric Kits - Manual (Page 5)

Quantity Shelf
Product Description Provided Storage Life
Components supplied in horseradish peroxidase kits
HRP color regent A (contains 4-chloro- 200 ml -20 °C 1 yr
1-naphthol in diethylene glycol)
HRP color reagents B (contains 10 ml 4 °C 1 yr
hydrogen peroxide)
HRP color development buffer, 10x 100 ml 4 °C >1 yr
Additional components
Citrate buffered saline (CBS), 3x, 100 g RT >1 yr
pH 5.5 (provided in the Protein G HRP
kit only)
* Each kit provides a different conjugate solution. These reagents are shipped
frozen, and can be stored at -20 °C until their initial use. Once thawed, store the
conjugate solution at 4 °C. Avoid repeated freeze-thaw cycles, which will cause
breakdown of the product.
Note: Each kit contains all reagents necessary to complete 200 x 5 ml
assays. An excess of conjugate has been supplied in each kit. BSA must
be substituted for gelatin when blocking the membrane in the Bio-Dot
or Bio-Dot SF microfiltration apparatus.
1.4 Safety Instructions
Read entire instruction manual before beginning the assay.
1. Wear gloves and protective clothing, such as a laboratory coat and
goggles, when preparing and working with the solutions in the
assay. DMF, diethylene glycol, 4-chloro-1-naphthol, and BCIP can
cause skin and eye irritation, and contact should be avoided. In case
of contact, immediately flush the skin or eyes with copious
amounts of water for at least 15 minutes, and remove contaminated
clothing.
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Catalog
Number
Product Description
Supported Nitrocellulose Membrane (0.2 µm)
162-0195 Sheets , 7 x 8.4 cm, 10
162-0196 Sheets , 15 x 15 cm, 10
162-0197 Roll , 30 cm x 3 m, 1
PVDF Membrane (0.2 µm)
162-0180 Sheets , 10 x 15 cm, 10
162-0181 Sheets , 15 x 15 cm, 10
162-0183 Sheets , 20 x 20 cm, 10
162-0184 Roll , 24 cm x 3.3 m, 1
162-0185 Sheets , 20 x 20 cm, 3
162-0186 Sheets , 7 x 8.4 cm, 10
1.3 Materials Provided with the Immun-Blot Kit
Storage and Stability of Kit Components
Quantity Shelf
Product Description Provided Storage Life
Components supplied in all kits
Tris buffered saline, 10x, pH 7.5 1 L 4 °C >1 yr
Gelatin, Blotting grade 50 g RT >1 yr
Tween-20, Blotting grade 5 ml RT >1 yr
Conjugate solution* 0.5 ml 4 °C 1 yr
Components supplied in alkaline phosphatase kits
AP color reagent A (contains NBT in 10 ml -20 °C 1 yr
aqueous dimethylformamide [DMF],
containing magnesium chloride)
AP color reagent B (contains 10 ml -20 °C 1 yr
BCIP in DMF)
AP color development buffer, 25x 40 ml 4 °C >1 yr
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