Bio-Rad Immun-Blot® AP Colorimetric Kits - Manual (Page 4)

Catalog
Number
Product Description
Individual Blotting Grade Reagents (continued)
170-6425 Protein G-HRP Conjugate
170-6435 Premixed Tris Buffered Saline, 10x, 1 L
170-6431 Horseradish Peroxidase Conjugate Substrate Kit
170-6432 Alkaline Phosphate Conjugate Substrate Kit
170-6537 Gelatin, Blotting Grade, 200 g
170-6531 Tween-20, Blotting Grade, 100 ml
Blotting Membranes
Nitrocellulose Membrane (0.45 µm)
162-0115 Roll, 33 cm x 3 m, 1
162-0145 Sheets, 7 x 8.4 cm, 10
162-0117 Sheets, 9 x 12 cm, 10
162-0114 Sheets, 15 x 9.2 cm, 10
162-0116 Sheets, 15 x 15 cm, 10
162-0113 Sheets, 20 x 20 cm, 5
Nitrocellulose Membrane (0.2 µm)
162-0112 Roll, 33 cm x 3 m, 1
162-0146 Sheets, 7 x 8.4 cm, 10
162-0147 Sheets, 13.5 x 16.5 cm, 10
Blotting Media
Supported Nitrocellulose Membrane (0.45 µm)
162-0190 Sheets , 7 x 8.4 cm, 10
162-0191 Sheets , 10 x 15 cm, 10
162-0192 Sheets , 15 x 15 cm, 10
162-0193 Sheets , 20 x 20 cm, 10
162-0194 Roll , 30 cm x 3 m, 1
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washed to remove unbound antibody and incubated with the respective
GAR, GAM, or GAH second antibody, or Protein A or Protein G,
which has been conjugated to alkaline phosphatase (AP) or horseradish
peroxidase (HRP). In the case of AP, a color development reagent
containing 5-bromo-4-chloro-3-indoyl phosphate (BCIP) and nitroblue
tetrazolium (NBT) yields purple bands or “dots” on a white
background. The HRP color development reagent, containing 4-chloro-
1-naphthol and hydrogen peroxidase, also yields purple bands against a
white background.
1.2 Product Information
Catalog
Number
Product Description
Immun-Blot Assay Kit
170-6460 Goat Anti-Rabbit IgG (H+L) AP
170-6461 Goat Anti-Mouse IgG (H+L) AP
170-6462 Goat Anti-Human IgG (H+L) AP
170-6463 Goat Anti-Rabbit IgG (H+L) HRP
170-6464 Goat Anti-Mouse IgG (H+L) HRP
170-6465 Goat Anti-Human IgG (H+L) HRP
170-6466 Protein A-HRP
170-6467 Protein G-HRP
Individual Blotting Grade Reagents
170-6518 Goat Anti-Rabbit IgG (H+L) AP Conjugate
170-6520 Goat Anti-Mouse IgG (H+L) AP Conjugate
170-6521 Goat Anti-Human IgG (H+L) AP Conjugate
170-6515 Goat Anti-Rabbit IgG (H+L) HRP Conjugate
170-6516 Goat Anti-Mouse IgG (H+L) HRP Conjugate
172-1050 Goat Anti-Human IgG (H+L) HRP Conjugate
170-6522 Protein A-HRP Conjugate
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