3 maintenance schedule, 4 electrode replacement – Bio-Rad ReadySub-Cell GT Cells User Manual

Page 16

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Alcohols

Methanol

Ethanol

Isopropyl alcohol

Do not use abrasive or highly alkaline cleaners on Sub-Cell GT parts.

Do not expose Sub-Cell GT parts to temperatures >60°C. Do not sterilize Sub-Cell GT parts by autoclaving
or dry heat.

4.3 Maintenance Schedule

Item

Look For

Frequency

Action

All parts

Dried salts, agarose, Each use

Clean parts as described in

grease, and dirt

Section 4.1

Electrical cables

Breaks or fraying

Each use

Replace cables

Trays

Chips or cracks

Each use

Replace tray

Electrode wires

Breaks

Each use

See Section 4.4 (Electrode

assembly replacement)

Cable connections

Looseness

Weekly

Replace banana jacks or

(banana jacks and plugs)

banana plug holders

GT base

Crazing, cracks

Monthly

Replace GT base

4.4 Electrode Replacement

The Sub-Cell GT systems allow easy replacement of broken electrode wires by removing the banana plug/
electrode wire assembly and ordering a new electrode assembly from Bio-Rad (Figure 4.1). Order the new
assembly using the part description and catalog numbers listed in Section 6, Product Information.

1.

Remove the broken wire assembly by placing one finger on the banana plug, and by pressing with
another finger the QuickSnap

feature on the outside of the upper part of the electrode wire assembly

and lifting upward. Discard the broken assembly.

2.

Insert the new assembly into the electrode assembly chamber of the GT base. Make sure the tab of the
QuickSnap

is properly seated in the slot of the electrode assembly chamber.

Banana plug

Electrode wire

Fig. 4.1. Removal of banana plug/electrode wire assembly.

Banana plug/
Electrode wire
assembly

Electrode
assembly
chamber

12

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