Bio-Rad ReadySub-Cell GT Cells - Manual (Page 8)

1.4 Specifications
Sub-Cell GT Wide Mini-Sub Mini-Sub Cell
System Cell GT System GT System
GT base footprint (L x W x H) 40.5 x 18 x 9.4 cm 25.5 x 17.8 x 6.8 cm 25.5 x 9.2 x 5.6 cm
GT base buffer volume = 1,500—2,000 ml 650—900 ml 265—320 ml
GT base gel size 15 x 15 cm 15 x 7 cm 7 x 7 cm
Gel tray sizes 15 x 10 cm 15 x 7 cm 7 x 7 cm
15 x 15 cm 15 x 10 cm 7 x 10 cm
15 x 20 cm
15 x 25 cm
Construction
GT base
Molded clear plastic
Gel casting gates Aluminum
Safety lid Molded clear plastic
Electrode wire guard Molded polycarbonate
Banana plugs Gold-plated brass, 4.4 cm length
Electrodes Platinum, 0.25 mm diameter
Electrical cables Dual, 20 AWG, tinned copper wire cable
Flame-retardant polyurethane insulation jacket
Electrical leads Nickel silver
Gel tray UV-transparent acrylic plastic (UVTP)
Combs Molded plastic and machined acrylic
Gel casting device Polycarbonate
0.64 cm silicon foam
= GT base buffer volumes will vary depending on the size and thickness of the gel used.
Section 2
Operating Instructions
Note: See Section 3, Gel and Electrophoresis Reagent Preparation, for information on the preparation of
RNA gels. See References 1 and 2 for more information on DNA and RNA electrophoresis.
2.1 DNA Gel Preparation
DNA agarose gels can be used to separate and visualize DNA of various sizes. Before casting an agarose gel,
consult Table 2.1, page 5, to determine the appropriate percent agarose gel to use, based on the size of DNA
to be separated.
Procedure
1. Determine the amount of agarose (grams) required to make the desired agarose gel concentration
and volume. Use Tables 2.1 and 2.2, page 5, as a guide for agarose concentration and gel volume
requirements.
Example: For a 1% agarose gel, add 1 gram of agarose to 100 ml of 1x electrophoresis buffer.
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