Bio-Rad ReadySub-Cell GT Cells - Manual (Page 5)

Section 1
General Information
1.1 Introduction
The Sub-Cell ® GT instruments (basic Sub-Cell ® GT cell, Wide Mini-Sub ® Cell GT, and Mini-Sub Cell ®
GT) comprise a comprehensive and flexible gel electrophoresis system that effectively separates nucleic
acids using submerged agarose gels. Submarine agarose gels are easy to cast and readily dissipate heat.
These gels allow sample underlaying and prevent electrical field discontinuities caused by wicks or sample
well dehydration. Agarose gels are ideal for the separation of DNA restriction digestions, polymerase chain
reaction (PCR*)-amplified fragments, and genomic DNA and RNA prior to Southern or northern blotting.
If operated correctly, agarose gel submarine electrophoresis can effectively separate nucleic acids from
20 base pairs to 20 kilobase pairs in length.
The Sub-Cell GT systems are designed for years of reproducible and rigorous use. These rugged
systems incorporate many features that make casting and running agarose gels simple and efficient.
The gel caster provides tape-free gel casting in trays. Gels can also be cast in the GT bases using specially
designed casting gates. Replaceable electrode cassettes provide a simple way to replace electrode wires.
A comprehensive assortment of base and tray sizes, including a variety of preparative, analytical, and
multichannel pipet compatible combs, makes these systems ideal for any agarose gel application.
Note: This manual contains instructions for the Sub-Cell GT electrophoresis systems only. Prior to the
release of the Sub-Cell GT systems, Bio-Rad supplied similar agarose gel electrophoresis cells: the original
Sub-Cell DNA electrophoresis cell, Wide Mini-Sub cell, and Mini-Sub cell systems. This manual does not
provide information on these earlier versions. Contact your local Bio-Rad representative for information
concerning the original Sub-Cell systems.
Definition of Symbols
Caution, risk of electrical shock Caution (refer to accompanying documents)
1.2 Safety
The Sub-Cell GT electrophoresis systems are designed for maximum user safety. The buffer chambers
are made of 2.75 mm thick injection-molded acrylic to create a leak-free electrophoresis environment.
The safety lids surround the buffer chamber to protect the user from exposure to electrical currents.
All Sub-Cell GT systems were designed for indoor use only.
Before use, inspect the GT base for cracks or chips, which may allow the buffer to leak from the base
and cause a potential electrical hazard. Additionally, inspect all electrical cables, banana jacks, and plugs for
loose connections, cracks, breaks, or corrosion. Do not use any part that is cracked, charred, or corroded.
These parts may also cause a potential electrical hazard. Contact your local Bio-Rad representative before
using a part that may be considered hazardous.
During electrophoresis, inspect the base and workbench for any signs of buffer leakage. If leaking buffer
is detected, disconnect the power to the cell immediately and contact your local Bio-Rad representative.
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