Bio-Rad CHEF-DR II System User Manual
Page 21
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![background image](/manuals/600269/21/background.png)
Example Calculations:
Mammalian:
(A)
(B)
(C)
(D)
Bacterial:
(A)
(B)
(C)
(D)
18
(6 x 10
9
bp)(660 g/mole) = 6.547 x 10
-12
g DNA/cell
6.02 x 10
23
bp/mole
(6.578 x 10
-12
g/cell)(5 x 10
7
cells/ml) =
(3.289 x 10
-4
g DNA/ml)(1x10
6
µg/g) - 329 mg DNA/ml
(329 µg DNA/ml)(1.2 genome equivalents)
≅
(394 µg DNA/ml)
394 µg DNA/ml
≅
40 µg DNA/plug
≅
20 µg DNA/lanes
10 plugs/ml
2 lanes/plug
(4.5 x 10
6
bp)(660 g/mole)
= 4.933 x 10
-15
g DNA/cell
6.02 x 10
23
bp/mole
(4.933 x 10
-15
g DNA/cell)(5 x 10
8
cells/ml) =
(2.467 x 10
-6
g DNA/ml)(1x10
6
µg/g) = 2.5 µg DNA/ml
(2.5 µg DNA/ml)(2.5 genome equivalents)
≅
(6.25 µg DNA/ml)
6.25 µg DNA/ml
≅
0.625 µg DNA/plug
≅
0.3 µg DNA/lane
10 plugs/ml
2 lanes/plug
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