Bio-Rad CHEF-DR II System - Manual (Page 6)

Accessories included
Variable speed pump 120 V, ground isolated. Flow rate 0.75 liter/min, typical
Casting stand 14 cm x 13 cm
Comb 10 well comb and comb holder
Tygon tubing 365 cm
Sample plug mold 10 slot
Yeast DNA Standard S. cerevisiae YNN295, 2 plugs
Chromosomal Grade Agarose 5 grams
Pulsed Field Certified Agarose 5 grams
Leveling bubble 1
Fuses 0.5 Amp Fast Blow, 2
Manual 1
Model 1000 Mini Chiller (Optional)
Weight 14 kg
Construction Aluminum
Dimensions 42 cm long x 23 cm wide x 24 cm high
Cooling capacity 75 watts of input power at 14 °C
Operating range 5 °C - 25 °C
Fuses 3.0 Amp Slow-Blow (100/120 V) or
1.5 Amp Slow-Blow (220/240 V)
Note: This equipment has been tested and found to comply with the limits for a Class A
digital device, pursuant to Part 15 of the FCC rules. These limits are designed to provide
reasonable protection against harmful interference when the equipment is operated in a
commercial environment. This equipment generates, uses, and can radiate radio frequency
energy and, if not installed and used in accordance with the instruction manual, may cause
harmful interference to radio communications. Operation of this equipment in a residen-
tial area is likely to cause harmful interference in which case the user will be required to
correct the interference at his own expense.
1.4 Description of Major Components
Drive Module
The drive module is a separate electronic device which distributes and maintains the indi-
vidual voltages for the 24 electrodes in the electrophoresis cell. It contains the electrode drivers
which provide and maintain the clamped homogeneous electric fields in the electrophoresis
cell. This feature, dynamic regulation (US Patent 4,878,008 issued to Bio-Rad Laboratories),
modulates the potentials so that the proper voltages are maintained regardless of gel size, or
fluctuations in buffer conductivity or temperature.
Control Module
The control module contains the both the power supply function, which provides the volt-
age for the 24 drivers in the drive module, and the switcher function, which alternates the
electric field in the electrophoresis cell. All run conditions, including initial and final switch
time (ramp), voltage gradient, and run time, are programmed directly into the control module.
In addition, up to 2 consecutively executing blocks of run conditions can be programmed,
increasing the variety of possible separations. The power supply outputs a maximum voltage
gradient of 6 V/cm, or 200 V. The lowest gradient is 0.6 V/cm, or 20 V.
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